Journal: Scientific Reports

Article Title: Piezo1 activation protects against sepsis-induced myocardial dysfunction in a pilot study

doi: 10.1038/s41598-025-00829-2

Figure Lengend Snippet: Piezo1 was upregulated in SIMD mice. A mouse model of sepsis was induced by intraperitoneal injection of LPS (15 mg/kg). (A) Representative M-mode echocardiographic images 12 h post-LPS administration. Scale bar = 100 ms/2 mm. (B-C) Quantitative analysis of LVEF% and LVFS%. (D) Quantitative analysis of Heart rate. (E) Plasma cardiac cTnT levels. Beta-tubulin served as a loading control. (F) Representative HE stained heart sections. Scale bar = 50 μm. (G) Western blot analysis of Piezo1 expression in mouse heart tissue. Beta-tubulin served as a loading control. Data were presented as mean ± SEM. P < 0.05 was considered statistically significant. Blots are representative of n = 6 biological replicates.

Article Snippet: C57BL/6 N female mice (10 weeks old) were obtained from Vital River Laboratory (Beijing, China), while age-matched Piezo1 ΔCM and Piezo1 flox/flox female mice were procured from Cyagen Biosciences, Inc (Jiangsu, China).

Techniques: Injection, Clinical Proteomics, Control, Staining, Western Blot, Expressing

Journal: Scientific Reports

Article Title: Piezo1 activation protects against sepsis-induced myocardial dysfunction in a pilot study

doi: 10.1038/s41598-025-00829-2

Figure Lengend Snippet: Piezo1 agonist Yoda1 ameliorated cardiac dysfunction in SIMD mice. Mice were pretreated with Yoda1 1 h before LPS (15 mg/kg) administration (A) Representative M-mode echocardiographic images 12 h post-LPS injection. Scale bar = 100 ms/2 mm. (B-C) Quantitative analysis of LVEF% and LVFS%. (D) Quantitative analysis of Heart rate. (E) Plasma cTnT levels. (F) Representative HE stained heart sections. Scale bar = 50 μm. Data were presented as mean ± SEM. P < 0.05 was considered statistically significant.

Article Snippet: C57BL/6 N female mice (10 weeks old) were obtained from Vital River Laboratory (Beijing, China), while age-matched Piezo1 ΔCM and Piezo1 flox/flox female mice were procured from Cyagen Biosciences, Inc (Jiangsu, China).

Techniques: Injection, Clinical Proteomics, Staining

Journal: Scientific Reports

Article Title: Piezo1 activation protects against sepsis-induced myocardial dysfunction in a pilot study

doi: 10.1038/s41598-025-00829-2

Figure Lengend Snippet: Piezo1 inhibitor GsMTx-4 aggravated cardiac dysfunction in SIMD mice. Mice were pretreated with GsMTx-4 1 h before LPS administration (15 mg/kg). (A) Representative M-mode echocardiographic images 12 h post-LPS injection. Scale bar = 100 ms/2 mm. (B-C) Quantitative analysis of LVEF% and LVFS%. (D) Quantitative analysis of Heart rate. (G) Plasma cTnT levels. (F) Representative HE stained heart sections. Scale bar = 50 μm. Data were presented as mean ± SEM. P < 0.05 was considered statistically significant.

Article Snippet: C57BL/6 N female mice (10 weeks old) were obtained from Vital River Laboratory (Beijing, China), while age-matched Piezo1 ΔCM and Piezo1 flox/flox female mice were procured from Cyagen Biosciences, Inc (Jiangsu, China).

Techniques: Injection, Clinical Proteomics, Staining

Journal: Scientific Reports

Article Title: Piezo1 activation protects against sepsis-induced myocardial dysfunction in a pilot study

doi: 10.1038/s41598-025-00829-2

Figure Lengend Snippet: Cardiomyocyte-specific Piezo1 deficiency exacerbated cardiac dysfunction in SIMD mice. Both Piezo1 ΔCM and Piezo1 flox/flox mice were subjected to LPS (15 mg/kg) injection. (A) Representative M-mode echocardiographic images 12 h post-LPS administration. Scale bar = 100 ms/2 mm. (B-C) Quantitative analysis of LVEF% and LVFS%. (D) Quantitative analysis of Heart rate. (E) Plasma cTnT levels. (F) Agarose gel electrophoresis of PCR products for Piezo1 genotyping. (G) Representative HE stained heart sections. Scale bar = 50 μm. (H) Western blot analysis of DUSP3 in mouse heart tissue. GAPDH served as a loading control. Data were presented as mean ± SEM. P < 0.05 was considered statistically significant. S denoted mice was treated with normal saline. LPS denoted mice was treated with LPS. ns stood for no statistical difference between the two groups. Blots are representative of n = 6 biological replicates.

Article Snippet: C57BL/6 N female mice (10 weeks old) were obtained from Vital River Laboratory (Beijing, China), while age-matched Piezo1 ΔCM and Piezo1 flox/flox female mice were procured from Cyagen Biosciences, Inc (Jiangsu, China).

Techniques: Injection, Clinical Proteomics, Agarose Gel Electrophoresis, Staining, Western Blot, Control, Saline

Journal: International Journal of Molecular Sciences

Article Title: Siponimod Modulates the Reaction of Microglial Cells to Pro-Inflammatory Stimulation

doi: 10.3390/ijms232113278

Figure Lengend Snippet: Siponimod modulates microglial cytokine expression and secretion in an inflammatory milieu. ( A , C , E , G ) quantitative RT-PCR analysis of Tnf ( A ), Ifnb ( C ), Il1b ( E ) and Il10 ( G ) gene expression after stimulation for 1 day with +/− 50 µM siponimod +/− LPS. ( B , D , F , H ) Quantification of TNF-α ( B ), IFNβ ( D ), IL-1β ( F ) and IL-10 ( H ) protein concentration in the cell culture medium of microglial cells stimulated for 3 days with +/− 50 µM siponimod +/− LPS using respective quantitative sandwich ELISA assays. Grey-dotted lines indicate the lowest standard of the ELISA kit: TNFα = 82.3 pg/mL, IFNβ = 15.63 pg/mL, IL-1β = 68.59 pg/mL and IL-10 = 8.23 pg/mL. Data are presented as mean values ± SEM. Grey dots represent individual data points. Significance of gene expression analysis as well as ELISA was assessed by 1-way analysis of variance (ANOVA) followed by Tukey’s post hoc test using Graph-Pad Prism 8.4.3 (GraphPad Software, San Diego, CA, USA). The experimental groups were considered significantly different at ** p < 0.01, *** p < 0.001.

Article Snippet: On the day of analysis, all reagents were thawed and adjusted to RT before the culture medium was measured in duplets using the following colorimetric sandwich ELISA kits according the manufacturers protocol: rat TNF alpha ELISA Kit (ab100785, Abcam), rat IL-1 beta ELISA kit (ab100768, abcam), rat IL-10 ELISA Kit (ab100764, abcam), Rat IFN-beta ELISA Kit (NBP3-06753, Novus Biologicals).

Techniques: Expressing, Quantitative RT-PCR, Gene Expression, Protein Concentration, Cell Culture, Sandwich ELISA, Enzyme-linked Immunosorbent Assay, Software